Increasing the yield of primary monolayer tissue culture by reusing a given cell suspension.

on quantitation by use of a hemacytometer. Depending upon the types of cells used and the experience of the workers in various laboratories, the dilutions of the packed volumes of cells may range from 1:100 to 1:600. After 3 to 5 days of incubation of the initial cell suspension, the usual subsequent steps are the removal and discarding of all unattached cells and replacement of the original medium with fresh growth medium (GM)